Dog IgG isotype control (HRP)
Quick Overview for Dog IgG isotype control (HRP) (ABIN964233)
Target
Conjugate
Application
Isotype
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Host
- Dog
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Supplier Product No.
- 004-0302
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Supplier
- Rockland
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Purpose
- Dog IgG Whole Molecule Peroxidase Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Dog IgG and anti-Dog Serum.
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Characteristics
- Dog IgG Peroxidase conjugated, Dog IgG HRP conjugated
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Application Notes
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Application Note: DOG IgG whole molecule Peroxidase conjugated can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: None -
Preservative
- Without preservative
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Target
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