Human IgG isotype control (FITC)
Quick Overview for Human IgG isotype control (FITC) (ABIN964315)
Target
Fragment
Conjugate
Application
Isotype
-
-
Host
- Human
-
Supplier Product No.
- 009-0203
-
Supplier
- Rockland
-
Purpose
- Human IgG Fc Fragment Fluorescein Conjugated
-
Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.
-
Characteristics
- Human IgG F(c) fragment fluorescein conjugation, Human IgG F(c) fragment FITC conjugated
-
Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
-
Labeling Ratio
- 3.2
-
-
Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
-
-
Application Notes
-
Application Note: Human IgG Fc Fluorescein conjugation is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
-
Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
-
Concentration
- 1.0 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Expiry Date
- 12 months
-
-
- IgG
-
Target Type
- Antibody
-
Background
- Background: Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Target
-
