Mouse IgG isotype control (FITC)
Quick Overview for Mouse IgG isotype control (FITC) (ABIN964337)
Target
Conjugate
Application
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-0202
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Supplier
- Rockland
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Purpose
- Mouse IgG Whole Molecule Fluorescein Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse IgG and anti-Mouse Serum.
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Characteristics
- fluorescein conjugated Mouse IgG, FITC conjugated Mouse IgG Whole molecule
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 4.1
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Application Notes
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Application Note: Mouse IgG Fluorescein has been tested by SDS-Page and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
Target
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