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Rabbit IgG Isotype Control

ELISA, WB, IHC IgG unconjugated
Rockland
Catalog No. ABIN964355
Supplier Product No.: 011-001-297

Quick Overview for Rabbit IgG Isotype Control (ABIN964355)

Target

IgG

Application

ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype

IgG
  • Host

    • 135
    • 124
    • 124
    • 84
    • 82
    • 58
    • 55
    • 52
    • 46
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 10
    • 8
    • 8
    • 7
    • 3
    • 2
    Rabbit

    Supplier Product No.

    011-001-297

    Supplier

    Rockland

    Purpose

    Low Endotoxin Control Rabbit IgG

    Characteristics

    Rabbit immunoglobulin G, Low EU Immunoglobulin control, Low EU standard

    Purification

    Low Endotoxin Control Rabbit IgG is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology.

    Sterility

    Sterile filtered

    Endotoxin Level

    Low Endotoxin
  • Application Notes

    Immunohistochemistry Dilution: User Optimized

    Application Note: Low Endotoxin Control Rabbit IgG has been tested in ELISA and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Neutralization Dilution: User Optimized

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Low Endotoxin Control Rabbit IgG is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Expiry Date

    6 months
  • Skowron-Kandzia, Tomsia, Koryciak-Komarska, Plewka, Wieczorek, Czekaj: "Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332-A Preliminary Study." in: Frontiers in medicine, Vol. 8, pp. 719899, (2021) (PubMed).

    Leiendecker, Jung, Krecioch, Neumann, Schleiffer, Mechtler, Wiesner, Obenauf: "LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma." in: EMBO molecular medicine, pp. e12525, (2020) (PubMed).

    Shan, Xu, Bleyer, Becker, Melbaum, Wemheuer, Hirschfeld, Wacker, Zhao, Schütz, Brenig: "Association of α/β-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid Composition." in: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).

    Aeckerle, Drummer, Debowski, Viebahn, Behr: "Primordial germ cell development in the marmoset monkey as revealed by pluripotency factor expression: suggestion of a novel model of embryonic germ cell translocation." in: Molecular human reproduction, Vol. 21, Issue 1, pp. 66-80, (2015) (PubMed).

  • Target

    IgG

    Target Type

    Antibody

    Background

    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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