Rat IgG isotype control (Agarose Beads)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Host
- Rat
- Conjugate
- Agarose Beads
- Application
- Immunoprecipitation (IP)
- Supplier Product No.
- 012-0051
- Supplier
- Rockland
- Purpose
- Rat IgG F(ab')2 Fragment Agarose Conjugated
- Characteristics
- Rat IgG F(ab')2 fragment Agarose Conjugate, Rat IgG Fab2 Agarose
- Purification
- This product is an purified Rat IgG isolated from normal Rat Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 of anti-Rat IgG.
- Isotype
- IgG
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- Application Notes
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Application Note: Rat IgG F(ab')2 Agarose is suitable for immunoprecipitation and Immunoaffinity purifications.
Immunoprecipitation Dilution: User Optimized
- Restrictions
- For Research Use only
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- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. DO NOT FREEZE.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Background: Rat IgG F(ab')2 Fragment Agarose Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Rat IgG F(ab')2 Molecules lack the Fc portion of Rat IgG and therefore receptors that bind Rat IgG Fc will not bind Rat IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by agarose conjugation.
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