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Rat IgG Isotype Control

Rockland 012-0103 ELISA, WB, SDS, IHC IgG unconjugated Fc fragment
Rockland
Catalog No. ABIN964381
Supplier Product No.: 012-0103
  • Target See all IgG products
    IgG
    Fragment
    Fc fragment
    Host
    • 136
    • 126
    • 118
    • 84
    • 81
    • 55
    • 53
    • 46
    • 44
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 10
    • 8
    • 7
    • 6
    • 4
    • 2
    Rat
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)
    Supplier Product No.
    012-0103
    Supplier
    Rockland
    Purpose
    Rat IgG Fc Fragment
    Cross-Reactivity (Details)
    Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c).  No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
    Characteristics
    Rat Immunoglobulin G F(c) fragment, IgG Fc
    Purification
    Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. 
    Sterility
    Sterile filtered
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: User Optimized

    Application Note: Rat IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. Stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site.
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