Quick Overview for Mouse IgG2a isotype control (PE) (ABIN964466)
Target
Clonality
Conjugate
Application
Clone
Isotype
-
-
Host
- Mouse
-
Supplier Product No.
- 010-0841
-
Supplier
- Rockland
-
Purpose
- Mouse IgG2a Isotype Control Phycoerythrin Conjugated
-
Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG2a was proven by Dot Blot.
-
Characteristics
- Phycoerythrin conjugated Mouse IgG2a isotype control, Mouse IgG2a subclass isotype control PE conjugation
-
Purification
- This product was prepared from ascitic fluid by affinity chromatography.
-
-
Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a APC
Mouse IgG2a isotype control (PerCP)FACS, IsoC Monoclonal MOPC-173 IgG2a PerCP
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a unconjugated
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a unconjugated
Rat IgG2a isotype control (FITC)FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (FITC)FACS, IsoC Monoclonal MOPC-173 IgG2a FITC
Mouse IgG2a isotype control (Biotin)FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a Biotin
Mouse IgG2a isotype control (PE)FACS, IsoC Monoclonal MOPC-173 IgG2a PE
Mouse IgG2a isotype control (FITC)FACS, ELISA Monoclonal HOPC-1 IgG2a FITC
-
-
Application Notes
-
Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: 1:1000-1:5000
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
-
Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
-
Concentration
- 1.0 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C
-
Storage Comment
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
-
Expiry Date
- 12 months
-
-
- IgG2a
-
Target Type
- Antibody
-
Background
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
-
