Kinase Assay Kit

Catalog No. ABIN1000318

Quick Overview for Kinase Assay Kit (ABIN1000318)

Target: Kinase

Application: Activity Assay (AcA)

Sample Type: Cell Extracts

Product Details

Specificity: 0.01 U/L

Characteristics: Safe. Non-radioactive assay.
Sensitive and accurate. As low as 0.01 U/L kinase can be quantified.
Homogeneous and convenient. Mix-incubate-measure type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature.
Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z' factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day.

Components: Reagent A: 10 mL. Reagent B: 10 mL. Assay Buffer: 25 mL. Standard: 100 µL 3 mM ADP.

Application Details

Application Notes: Kinase activity assays and HTS for kinase inhibitors. ADP determination in cells and other biological samples.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Target Details

Target: Kinase

Background: Rapid mix-and-measure procedure for determination of kinase activity and high-throµghput screening for kinase inhibitors by fluorimetric (530nm/590nm) method.
Procedure: 10 min.

Kinases, also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug developments. This Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorimetric (530nm/590nm) assay method.