Immunoglobulin isotyping

Catalog No. ABIN1113186

Quick Overview for Immunoglobulin isotyping (ABIN1113186)

Target: Ig

Reactivity: Mouse

Detection Method: Colorimetric

Application: Isotyping (IsoT)

Sample Type: Cell Culture Supernatant, Purified Protein

Product Details

Binding Specificity: Heavy & Light Chain

Conjugate: HRP

Purpose: The method employs the quantitative sandwich enzyme immunoassay technique.

Brand: FastELISA®

Specificity: Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,

Characteristics: The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.

Application Details

Comment:

One Step ELISA 30 min total time

Assay Time: < 1 h

Plate: Pre-coated

Protocol: Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Reagent Preparation:

dilution

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Ig

Alternative Name: Immunoglobulins

Background: Isotyping of mouse immunoglobulins