Ig ELISA Kit

Catalog No. ABIN4993802

The Mouse Ig ELISA Kit (ABIN4993802) is a Colorimetric ELISA Kit designed to quantify Mouse Ig.

Quick Overview for Ig ELISA Kit (ABIN4993802)

Target: Ig

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details Ig ELISA Kit

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Ig Isotyping.

Analytical Method: Quantitative

Specificity: IgG

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for Ig

Target: Ig

Background: Antibody isotyping is a critical and beneficial aspect of hybridoma development. This kit can identify six immunoglobulin heavy chain isotypes and two light chain isotypes in mice: IgA, IgM, IgG1, IgG2a, IgG2b, IgG3, kappa chain and lambda chain. It can accurately and specifically identify which heavy and light chain of the hybridoma samples is producing and if it is monoclonal or not. This kit is a powerful tool to isolate and characterize each potential clone.