Cortisol ELISA Kit
Quick Overview for Cortisol ELISA Kit (ABIN1326818)
Target
See all Cortisol ELISA KitsReactivity
Detection Method
Method Type
Application
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Purpose
- The Cortisol is a solid phase competitive ELISA. The samples and Cortisol enzyme conjugate are added to the wells coated with anti-Cortisol monoclonal antibody. Cortisol in the patient's sample competes with a Cortisol enzyme conjugate for binding sites. Unbound Cortisol and Cortisol enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the samples. A standard curve is prepared relating color intensity to the concentration of the Cortisol.
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Analytical Method
- Quantitative
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Cortisol ELISA Kit
Reactivity: Various Species Colorimetric Sandwich ELISA Fecal, Hair, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitReactivity: Various Species Colorimetric Sandwich ELISA 100 pg/mL - 3200 pg/mL Fecal, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA KitReactivity: Various Species Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReactivity: Mouse Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReactivity: Rabbit Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitSmall Sample Reactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitHigh Sensitivity Reactivity: Various Species Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitReactivity: Human Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReactivity: Monkey Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Cortisol
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Target Type
- Hormone
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Background
- Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found in the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTH-dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. This kit can detect low level of cortisol in sheep serum or plasma (less than 0.1 ngml).
Target See all Cortisol ELISA Kits
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