SSA IgG ELISA Kit

Catalog No. ABIN1326888

The Human SSA IgG ELISA Kit (ABIN1326888) is a Colorimetric ELISA Kit designed to quantify Human SSA IgG. This ELISA Kit has been cited in 1 publication.

Quick Overview for SSA IgG ELISA Kit (ABIN1326888)

Target: SSA IgG

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Serum

Product Details

Purpose: Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Analytical Method: Qualitative

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Rupasree, Naushad, Rajasekhar, Uma, Kutala: "Association of TLR4 (D299G, T399I), TLR9 -1486T>C, TIRAP S180L and TNF-? promoter (-1031, -863, -857) polymorphisms with risk for systemic lupus erythematosus among South Indians." in: Lupus, Vol. 24, Issue 1, pp. 50-7, (2014) (PubMed).

Target Details

Target: SSA IgG

Target Type: Antibody

Background: Systemic autoimmune disease is characterized by the presence of circulating auto-antibodies directed to a wide variety of cellular antigens.Systemic lupus erythematosis (SLE), commonly referred to as Lupus is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositisdermatomyositis.The majority can be diagnosed by clinical presentation and their antibody profiles to the various antigens involved, which include dsDNA, SM, RNP, SSA, SSB, Scl-70, Jo1 and Histones. Therefore, immunoassays for autoantibodies are useful for diagnostic and prognostic evaluations of autoimmune disease. SSA (Ro) antigen (60kd) and 52 kd polypeptides complexed with Ro RNAs are detected in about 75% of primary and secondary Sj_gren syndrome,In 90% of subacute cutaneous lupus and in the vasculitis-associated Sj_gren syndrome, SS-A autoantibodies are present and are accompanied in ~50% by SS-BLa autoantibodies. The coexistence of SSA and SSB autoantibodies probably reflects the presence of these polypeptides on the same particle and the spreading among autoimmunity to these self antigens.