Dystrophin ELISA Kit

Catalog No. ABIN1569434

Product Details Dystrophin ELISA Kit

Target: Dystrophin (DMD)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DMD in mouse tissue homogenates, cell lysates and other biological fluids.

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of this index.

Cross-Reactivity (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.108 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Material not included:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Sample Volume: 100 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Assay Procedure:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Expiry Date: 12 months

Target Details for Dystrophin

Target: Dystrophin (DMD)

Alternative Name: DMD (DMD Products)

Synonyms: DMD ELISA Kit, Dmd ELISA Kit, MGC83347 ELISA Kit, DKFZp459C1629 ELISA Kit, dmd ELISA Kit, MGC79631 ELISA Kit, DKFZp468A1620 ELISA Kit, Dp71 ELISA Kit, cb664 ELISA Kit, zfDYS ELISA Kit, im:6911785 ELISA Kit, zgc:110165 ELISA Kit, BMD ELISA Kit, CMD3B ELISA Kit, DXS142 ELISA Kit, DXS164 ELISA Kit, DXS206 ELISA Kit, DXS230 ELISA Kit, DXS239 ELISA Kit, DXS268 ELISA Kit, DXS269 ELISA Kit, DXS270 ELISA Kit, DXS272 ELISA Kit, DXSmh7 ELISA Kit, DXSmh9 ELISA Kit, Dp427 ELISA Kit, dys ELISA Kit, mdx ELISA Kit, pke ELISA Kit, DNADMD1 ELISA Kit, RATDMD ELISA Kit, CG17750 ELISA Kit, CG31175 ELISA Kit, CG34157 ELISA Kit, CG7240 ELISA Kit, CG7243 ELISA Kit, CG7344 ELISA Kit, DLP ELISA Kit, DLP1 ELISA Kit, DLP186 ELISA Kit, DLP2 ELISA Kit, DLP3 ELISA Kit, DmDLP ELISA Kit, DmDYS ELISA Kit, Dmel\\CG34157 ELISA Kit, Dp117 ELISA Kit, Dp186 ELISA Kit, Dp205 ELISA Kit, GI3046716 ELISA Kit, IDLP ELISA Kit, det ELISA Kit, dmDLP ELISA Kit, dmDp186 ELISA Kit, dmDys ELISA Kit, dystrophin ELISA Kit, dystrophin, gene 1 L homeolog ELISA Kit, dystrophin, gene 1 ELISA Kit, dystrophin, muscular dystrophy ELISA Kit, Dystrophin ELISA Kit, DMD ELISA Kit, LOC708073 ELISA Kit, dmd.1.L ELISA Kit, LOC465557 ELISA Kit, dmd.1 ELISA Kit, dmd ELISA Kit, Dmd ELISA Kit, Dys ELISA Kit

Background: Alternative name: CMD3B, Muscular Dystrophy,Duchenne And Becker Types

Gene ID: 13405

UniProt: P11531

Pathways: Skeletal Muscle Fiber Development