Angiopoietin 2 ELISA Kit

Catalog No. ABIN1672763

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Angiopoietin 2 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva and Milk. There is 1 publication available.

Quick Overview for Angiopoietin 2 ELISA Kit (ABIN1672763)

Target: Angiopoietin 2 (ANGPT2)

Binding Specificity: AA 19-496

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk

Product Details Angiopoietin 2 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Angiopoietin-2

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Y19-F496

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Y19-F496

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 fibrinogen C-terminal domain.

Plate: Pre-coated

Protocol: human Angiopoietin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(NSO, Y19-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiopoietin-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Angiopoietin-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.049, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 3.49, Standard deviation: 0.181, CV(%): 5.2
• Sample 3: n=16, Mean(ng/ml): 7.01, Standard deviation: 0.407, CV(%): 5.8,
• Sample 1: n=24, Mean(ng/ml): 1.05, Standard deviation: 0.071, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 3.32, Standard deviation: 0.236, CV(%): 7.1
• Sample 3: n=24, Mean(ng/ml): 6.67, Standard deviation: 0.5, CV(%): 7.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Angiopoietin 2 ELISA Kit (ABIN1672763)

You, Zhuge, Zhu, Si et al.: "Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with ..." in: International journal of ophthalmology, Vol. 7, Issue 4, pp. 648-53, (2014) (PubMed).

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: ANGPT2

Background:

Protein Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .

Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Synonyms: Angiopoietin-2,ANG-2,ANGPT2,

Full Gene Name: Angiopoietin-2

Cellular Localisation: Secreted.

Gene ID: 285

UniProt: O15123

Pathways: RTK Signaling