P-Cadherin ELISA Kit

Catalog No. ABIN1672767

This Mouse P-Cadherin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse P-Cadherin in Cell Culture Supernatant and Serum.

Quick Overview for P-Cadherin ELISA Kit (ABIN1672767)

Target: P-Cadherin (CDH3)

Binding Specificity: AA 100-647

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details P-Cadherin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse P-Cadherin

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: E100-G647

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E100-G647

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse P-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for P-Cadherin has been precoated onto 96-well plates. Standards(NSO, E100-G647) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Cadherin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse P-Cadherin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 433, Standard deviation: 27.28, CV(%): 6.3
• Sample 2: n=16, Mean(pg/ml): 1314, Standard deviation: 88.04, CV(%): 6.7
• Sample 3: n=16, Mean(pg/ml): 2658, Standard deviation: 180.74, CV(%): 6.8,
• Sample 1: n=24, Mean(pg/ml): 437, Standard deviation: 31.90, CV(%): 7.3
• Sample 2: n=24, Mean(pg/ml): 1356, Standard deviation: 103.06, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 2713, Standard deviation: 211.61, CV(%): 7.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for P-Cadherin

Target: P-Cadherin (CDH3)

Alternative Name: CDH3

Background:

Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Background: P-Cadherin, also known as Cadherin-3(CDH3), is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. Cells expressing CDH3 also adhered to one another more tightly than the parental cell line.

Synonyms: Cadherin-3,Placental cadherin,P-cadherin,Cdh3,Cdhp,

Full Gene Name: Cadherin-3

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

Gene ID: 12560

UniProt: P10287