DPP4 ELISA Kit

Catalog No. ABIN1672773

The Human DPP4 ELISA Kit (ABIN1672773) is a Colorimetric ELISA Kit designed to quantify Human DPP4. This ELISA Kit has been cited in 3 publications.

Quick Overview for DPP4 ELISA Kit (ABIN1672773)

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Binding Specificity: AA 34-766

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Product Details DPP4 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich Human CD26/DPP4 ELISA Kit to quantitate Human DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: D34-P766

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.5%, 6.4%, 6.2%
Inter-Assay Precision (CV%): 4.9%, 8.3%, 7.2%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for DPP4 ELISA Kit (ABIN1672773)

Boccardi, Marano, Rossetti, Rizzo, di Martino, Paolisso: "Serum CD26 levels in patients with gastric cancer: a novel potential diagnostic marker." in: BMC cancer, Vol. 15, pp. 703, (2016) (PubMed).

Violi, Loffredo, Pignatelli, Angelico, Bartimoccia, Nocella, Cangemi, Petruccioli, Monticolo, Pastori, Carnevale: "Extra virgin olive oil use is associated with improved post-prandial blood glucose and LDL cholesterol in healthy subjects." in: Nutrition & diabetes, Vol. 5, pp. e172, (2015) (PubMed).

Garcia-Segura, Muller, Dunant: "Increase in the number of presynaptic large intramembrane particles during synaptic transmission at the Torpedo nerve-electroplaque junction." in: Neuroscience, Vol. 19, Issue 1, pp. 63-79, (1986) (PubMed).

Target Details for DPP4

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Alternative Name: DPP4

Background:

Background: Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

Gene Full Name: dipeptidyl peptidase 4

Gene ID: 1803

UniProt: P27487

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity