BMP7 ELISA Kit

Catalog No. ABIN1672823

This Colorimetric ELISA kit is designed for the quantitative measurement of Human BMP7. There are 5 publications available.

Quick Overview for BMP7 ELISA Kit (ABIN1672823)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Binding Specificity: AA 293-431

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Osseous Tissue, Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Product Details BMP7 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-7

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S293-H431

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S293-H431

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: human BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been precoated onto 96-well plates. Standards(NSO, S293-H431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-7 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates, serum, plasma((heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-7 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 19.8, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 765, Standard deviation: 42.8, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 1108, Standard deviation: 57.6, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 372, Standard deviation: 30.5, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 780, Standard deviation: 62.4, CV(%): 8
• Sample 3: n=24, Mean(pg/ml): 1265, Standard deviation: 98.7, CV(%): 7.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for BMP7 ELISA Kit (ABIN1672823)

Wang, Xue, Zhao, Liu, Ma, Ma: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." in: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).

Li, Yi, Jin, Duan, Min: "Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration." in: American journal of translational research, Vol. 7, Issue 8, pp. 1417-28, (2015) (PubMed).

Qu, Du, Zhang, Li, Han, Qiu: "Changes in the expression of bone morphogenetic protein 7 and tamm- horsfall protein in the early stages of diabetic nephropathy." in: Nephro-urology monthly, Vol. 4, Issue 2, pp. 466-9, (2013) (PubMed).

Tu, Dong, Lu: "Effects of Panax notoginoside on the nephropathy in rats with type 1 diabetes mellitus." in: Chinese journal of integrative medicine, Vol. 17, Issue 8, pp. 612-5, (2011) (PubMed).

Che, Zhang, Li, Tan, Bai, Qu: "Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 18, Issue 4, pp. 496-503, (2010) (PubMed).

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP7

Background:

Background: Bone morphogenetic protein 7 or BMP7(also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Synonyms: Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ,cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ,cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1)(BMP7), mRNA ,BMP7 ,hCG_40100 ,

Full Gene Name: bone morphogenetic protein 7

Gene ID: 655

UniProt: A8K571

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance