Clusterin ELISA Kit

Catalog No. ABIN1672832

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Clusterin. There are 3 publications available.

Quick Overview for Clusterin ELISA Kit (ABIN1672832)

Target: Clusterin (CLU)

Binding Specificity: AA 23-227, AA 228-449

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Product Details Clusterin ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: Sandwich Human Clusterin ELISA Kit to quantitate Human CLU in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 20 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.1%, 5.8%, 4.2%
Inter-Assay Precision (CV%): 6.3%, 6.3%, 5%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for Clusterin ELISA Kit (ABIN1672832)

Kim, Song, Kim, Jeon, Kim, Kang, Chun, Hong, Chung, Kim, Kim, Kim: "Urine clusterin/apolipoprotein J is linked to tubular damage and renal outcomes in patients with type 2 diabetes mellitus." in: Clinical endocrinology, Vol. 87, Issue 2, pp. 156-164, (2018) (PubMed).

Song, Zhang, Zhang, Du, Guo, Kuang, Wang, Wu, Zou, Zou, Lv, Wang: "A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting." in: Scientific reports, Vol. 8, Issue 1, pp. 6394, (2018) (PubMed).

Zheng, Yao, Qian, Sai, Qiu, Yang, Wu, Dong, Yao: "Oncogenic secretory clusterin in hepatocellular carcinoma: Expression at early staging and emerging molecular target." in: Oncotarget, Vol. 8, Issue 32, pp. 52321-52332, (2017) (PubMed).

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: CLU

Background:

Background: Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several Synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Gene Full Name: clusterin

Gene ID: 1191

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling