DKK1 ELISA Kit

Catalog No. ABIN1672840

Mouse DKK1 ELISA Kit, Colorimetric assay for quantification of Mouse DKK1, that has been mentioned in 1 publication.

Quick Overview for DKK1 ELISA Kit (ABIN1672840)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Binding Specificity: AA 30-272

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details DKK1 ELISA Kit

Minimum Detection Limit: 46.9 pg/mL

Purpose: Sandwich Mouse DKK-1 ELISA Kit to quantitate Mouse Dkk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: S30-H272

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.6%, 7.1%, 4.8%
Inter-Assay Precision (CV%): 6.8%, 7.4%, 5.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for DKK1 ELISA Kit (ABIN1672840)

Sugatani, Agapova, Malluche, Hruska: "SIRT6 deficiency culminates in low-turnover osteopenia." in: Bone, Vol. 81, pp. 168-77, (2015) (PubMed).

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background:

Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Gene Full Name: dickkopf WNT signaling pathway inhibitor 1

Gene ID: 13380

UniProt: O54908

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation