L-Selectin ELISA Kit

Catalog No. ABIN1672850

This Colorimetric ELISA kit is designed for the quantitative measurement of Chemical L-Selectin.

Quick Overview for L-Selectin ELISA Kit (ABIN1672850)

Target: L-Selectin (SELL) (Selectin L (SELL))

Binding Specificity: AA 39-332

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625-40000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details L-Selectin ELISA Kit

Minimum Detection Limit: 625 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat sL-Selectin

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: W39-N332

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <20pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: W39-N332

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the selectin/LECAM family.

Plate: Pre-coated

Protocol: rat L-Selectin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for L-Selectin has been precoated onto 96-well plates. Standards(NSO, W39-N332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for L-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat L-Selectin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL,10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL rat L-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat L-Selectin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 5.3, Standard deviation: 0.18, CV(%): 3.4
• Sample 2: n=16, Mean(ng/ml): 12, Standard deviation: 0.516, CV(%): 4.3
• Sample 3: n=16, Mean(ng/ml): 29, Standard deviation: 1.653, CV(%): 5.7,
• Sample 1: n=24, Mean(ng/ml): 8.8, Standard deviation: 0.449, CV(%): 5.1
• Sample 2: n=24, Mean(ng/ml): 16.5, Standard deviation: 1.023, CV(%): 6.2
• Sample 3: n=24, Mean(ng/ml): 31.4, Standard deviation: 2.17, CV(%): 6.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: SELL

Background:

Protein Function: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .

Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Synonyms: L-selectin ,Sell ,rCG_45999 ,

Full Gene Name: L-selectin

Gene ID: 29259

UniProt: Q63762