MYD88 ELISA Kit

Catalog No. ABIN1672854

This Human MYD88 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human MYD88.

Quick Overview for MYD88 ELISA Kit (ABIN1672854)

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Binding Specificity: AA 1-296

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant

Product Details MYD88 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MyD88

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: M1-P296

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: M1-P296

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 death domain.

Tissue Specificity: Ubiquitous. .

Plate: Pre-coated

Protocol: human MyD88 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MyD88 has been precoated onto 96-well plates. Standards(E.coli, M1-P296) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MyD88 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MyD88 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MyD88 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MyD88 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 2.1, Standard deviation: 0.13, CV(%): 6.2
• Sample 2: n=16, Mean(ng/ml): 7, Standard deviation: 0.371, CV(%): 5.3
• Sample 3: n=16, Mean(ng/ml): 13.7, Standard deviation: 0.7, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 2.7, Standard deviation: 0.189, CV(%): 7
• Sample 2: n=24, Mean(ng/ml): 8.6, Standard deviation: 0.559, CV(%): 6.5
• Sample 3: n=24, Mean(ng/ml): 15.2, Standard deviation: 0.882, CV(%): 5.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Alternative Name: MYD88

Background:

Protein Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .

Background: Myeloid differentiation primary response gene(88)(MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9, the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.

Synonyms: Myeloid differentiation primary response protein MyD88,MYD88,

Full Gene Name: Myeloid differentiation primary response protein MyD88

Cellular Localisation: Cytoplasm.

Gene ID: 4615

UniProt: Q99836

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades