Myeloperoxidase ELISA Kit

Catalog No. ABIN1672855

Product Details Myeloperoxidase ELISA Kit

Target: Myeloperoxidase (MPO)

Binding Specificity: AA 16-718

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MPO/Myeloperoxidase

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: M16-T718

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: M16-T718

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse MPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MPO has been precoated onto 96-well plates. Standards(NSO, M16-T718) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MPO amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MPO standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1248, Standard deviation: 56.16, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 3528, Standard deviation: 179.93, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 6243, Standard deviation: 387.1, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 1425, Standard deviation: 84.1, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 4127, Standard deviation: 247.6, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 6785, Standard deviation: 468.2, CV(%): 6.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Myeloperoxidase ELISA Kit (ABIN1672855)

Chen, Yang, Hu, Cheng, Xu, Cai, Wang, Yang, Cao: "Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice." in: Oncotarget, Vol. 7, Issue 38, pp. 61643-61655, (2018) (PubMed).

Wu, He, Feng, Ye, Wang, Tan, Yu, Hu, Zheng, Zhou: "MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor." in: Oncotarget, Vol. 8, Issue 1, pp. 705-721, (2018) (PubMed).

Yang, Lai, Zheng, Kang, Xian, Wang, Shi, Meng, Li, Chen, Zhang: "A novel pH-controlled hydrogen sulfide donor protects gastric mucosa from aspirin-induced injury." in: Journal of cellular and molecular medicine, Vol. 21, Issue 10, pp. 2441-2451, (2018) (PubMed).

Chen, Yang, Liu, Teng, Ye, Xu, Cai, Cheng, Yang, Hu, Wang, Cao: "Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice." in: Journal of ethnopharmacology, Vol. 166, pp. 149-56, (2015) (PubMed).

Wang, Tang, Li, Wang: "Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 121-5, (2015) (PubMed).

Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

Chen, Chen, Qin: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO (MPO Products)

Synonyms: mKIAA4033 ELISA Kit, MPO ELISA Kit, LOC100335032 ELISA Kit, POX2' ELISA Kit, XPOX2' ELISA Kit, mpo ELISA Kit, mpo-A ELISA Kit, pmr-1 ELISA Kit, pmr1 ELISA Kit, pox2 ELISA Kit, xpox2 ELISA Kit, myeloperoxidase ELISA Kit, myeloperoxidase L homeolog ELISA Kit, MPO ELISA Kit, Mpo ELISA Kit, LOC100335032 ELISA Kit, mpo.L ELISA Kit

Background:

Protein Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (By similarity). .

Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Synonyms: Myeloperoxidase,MPO,1.11.2.2,Myeloperoxidase light chain,Myeloperoxidase heavy chain,Mpo,

Full Gene Name: Myeloperoxidase

Cellular Localisation: Lysosome.

Gene ID: 17523

UniProt: P11247

Pathways: Chromatin Binding