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Cathepsin S ELISA Kit

CTSS Reactivity: Human AA 17-331 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1672865
  • Target See all Cathepsin S (CTSS) ELISA Kits
    Cathepsin S (CTSS)
    Binding Specificity
    AA 17-331, AA 109-331
    Reactivity
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin S
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: Q17-I331&S109-I331
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q17-I331&S109-I331
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    human Cathepsin S ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin S has been precoated onto 96-well plates. Standards(NSO, Q17-I331 & S109-I331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin S is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin S amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin S standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin S standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 443, Standard deviation: 23.48, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 1215, Standard deviation: 65.61, CV(%): 5.4
    • Sample 3: n=16, Mean(pg/ml): 2611, Standard deviation: 151.44, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 455, Standard deviation: 27.76, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 1219, Standard deviation: 76.80, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 2659, Standard deviation: 178.15, CV(%): 6.7
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all Cathepsin S (CTSS) ELISA Kits
    Cathepsin S (CTSS)
    Alternative Name
    CTSS (CTSS Products)
    Synonyms
    ctss-a ELISA Kit, MGC69026 ELISA Kit, ctss-b ELISA Kit, MGC80097 ELISA Kit, ctss.1 ELISA Kit, CTSS ELISA Kit, ctss ELISA Kit, cats ELISA Kit, cathepsin S ELISA Kit, cathepsin S L homeolog ELISA Kit, cathepsin S S homeolog ELISA Kit, Cathepsin S ELISA Kit, CTSS ELISA Kit, Ctss ELISA Kit, ctss.L ELISA Kit, ctss.S ELISA Kit, ctss ELISA Kit, cats ELISA Kit
    Background

    Protein Function: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

    Background: Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization(FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

    Synonyms: Cathepsin S,3.4.22.27,CTSS,

    Full Gene Name: Cathepsin S

    Cellular Localisation: Lysosome.
    Gene ID
    1520
    UniProt
    P25774
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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