FSTL1 ELISA Kit

Catalog No. ABIN1672869

The Human FSTL1 ELISA Kit (ABIN1672869) is a Colorimetric ELISA Kit designed to quantify Human FSTL1. This ELISA Kit has been cited in 1 publication.

Quick Overview for FSTL1 ELISA Kit (ABIN1672869)

Target: FSTL1 (Follistatin-Like 1 (FSTL1))

Binding Specificity: AA 1-308

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details FSTL1 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich Human FSTL1/Tsc 36 ELISA Kit to quantitate Human FSTL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: M1-I308

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.5%, 7.4%, 4%
Inter-Assay Precision (CV%): 7.8%, 7.8%, 4.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for FSTL1 ELISA Kit (ABIN1672869)

Wang, Li, Xu, Tao, Zhu, Sun, Fan, Zhang, Dong, Yu: "Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis." in: Arthritis research & therapy, Vol. 13, Issue 6, pp. R193, (2012) (PubMed).

Target Details for FSTL1

Target: FSTL1 (Follistatin-Like 1 (FSTL1))

Alternative Name: FSTL1

Background:

Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Gene Full Name: follistatin like 1

Gene ID: 11167

UniProt: Q12841