Neurotrophin 4 ELISA Kit

Catalog No. ABIN1706065

Human Neurotrophin 4 ELISA Kit Colorimetric assay for quantification of Human Neurotrophin 4 and has been mentioned in 2+ publications.

Quick Overview for Neurotrophin 4 ELISA Kit (ABIN1706065)

Target: Neurotrophin 4 (NTF4)

Binding Specificity: AA 81-210

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Neurotrophin 4 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NT-4

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: sf21
Immunogen sequence: G81-A210

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: G81-A210

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

Plate: Pre-coated

Protocol: human NT-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NT-4 has been precoated onto 96-well plates. Standards(sf21, G81-A210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NT-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NT-4 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 248, Standard deviation: 11.16, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 745, Standard deviation: 38, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 78.31, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 280, Standard deviation: 14.84, CV(%): 5.3
• Sample 2: n=24, Mean(pg/ml): 821, Standard deviation: 46, CV(%): 5.6
• Sample 3: n=24, Mean(pg/ml): 1402, Standard deviation: 96.74, CV(%): 6.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Neurotrophin 4 ELISA Kit (ABIN1706065)

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Sun, Zhang, Li, Wang, Ma, Sun, Liu, Wang, Zhou: "Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts." in: Theriogenology, Vol. 78, Issue 1, pp. 39-48, (2012) (PubMed).

Target Details for Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Alternative Name: NTF4

Background:

Protein Function: Target-derived survival factor for peripheral sensory sympathetic neurons.

Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Synonyms: Neurotrophin-4,NT-4,Neurotrophin-5,NT-5,Neutrophic factor 4,NTF4,NTF5,

Full Gene Name: Neurotrophin-4

Cellular Localisation: Secreted.

Gene ID: 4909

UniProt: P34130

Pathways: RTK Signaling