MMP12 ELISA Kit

Catalog No. ABIN1706069

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MMP12. There are 3 publications available.

Quick Overview for MMP12 ELISA Kit (ABIN1706069)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Binding Specificity: AA 17-470

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details MMP12 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich Human MMP-12 ELISA Kit to quantitate Human MMP12 in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: L17-C470

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.4%, 4.1%, 5.8%
Inter-Assay Precision (CV%): 6.8%, 6%, 5.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for MMP12 ELISA Kit (ABIN1706069)

Bchir, Nasr, Bouchet, Benzarti, Garrouch, Tabka, Susin, Chahed, Bauvois: "Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease." in: Journal of cellular and molecular medicine, Vol. 21, Issue 7, pp. 1280-1291, (2018) (PubMed).

Narimiya, Wada, Kanzaki, Ishikawa, Tsuge, Yamaguchi, Nakamura: "Orthodontic tensile strain induces angiogenesis via type IV collagen degradation by matrix metalloproteinase-12." in: Journal of periodontal research, Vol. 52, Issue 5, pp. 842-852, (2018) (PubMed).

Kobayashi, Jokaji, Miyazawa-Hira, Takatsuka, Tanaka, Ooi, Nakamura, Kawashiri: "Elastin‑derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3147-3154, (2018) (PubMed).

Target Details for MMP12

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: MMP12

Background:

Background: Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

Gene Full Name: matrix metallopeptidase 12

Gene ID: 4321

UniProt: P39900