IL17F ELISA Kit

Catalog No. ABIN1706083

Rat IL17F ELISA Kit, Colorimetric assay for quantification of Rat IL17F, that has been mentioned in 1 publication.

Quick Overview for IL17F ELISA Kit (ABIN1706083)

Target: IL17F (Interleukin 17F (IL17F))

Binding Specificity: AA 19-153

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details IL17F ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Rat IL-17F ELISA Kit to quantitate Rat Il17f in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A19-A153

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.8%, 5.4%, 4.3%
Inter-Assay Precision (CV%): 7.2%, 6.7%, 5.7%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IL17F ELISA Kit (ABIN1706083)

McAllister, Henry, Kreindler, Dubin, Ulrich, Steele, Finder, Pilewski, Carreno, Goldman, Pirhonen, Kolls et al.: "Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 404-12, (2005) (PubMed).

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL17F

Background:

Background: Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.

Gene Full Name: interleukin 17F

Gene ID: 301291

UniProt: Q5BJ95

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity