SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit

Catalog No. ABIN1882430

Quick Overview for SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit (ABIN1882430)

Target: Caspase 3 (CASP3)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Characteristics: The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Component A from light and moisture. Keep Components A and B away from light.

Storage: -20 °C

Storage Comment: Store Components A, B, C, and E at -20 °C. For convenience, Components D and F can be stored at 4 °C.

References

Freise, Ruehl, Seehofer, Hoyer, Somasundaram: "The inhibitor of Ca(2+)-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB." in: Investigational new drugs, Vol. 31, Issue 2, pp. 452-7, (2013) (PubMed).

Meek, LeBoeuf, Saha, Alpers, Hudkins, Cooney, Anderberg, Tuttle: "Glomerular cell death and inflammation with high-protein diet and diabetes." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 28, Issue 7, pp. 1711-20, (2013) (PubMed).

Cheung, Revers, Perampalam, Wei, Kiarash, Green, Abdul-Wahid, Gariépy: "An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo." in: Molecular cancer, Vol. 9, pp. 28, (2010) (PubMed).

Freise, Erben, Neuman, Kim, Zeitz, Somasundaram, Ruehl: "An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes." in: The Journal of nutritional biochemistry, Vol. 21, Issue 12, pp. 1170-7, (2010) (PubMed).

Ruehl, Erben, Kim, Freise, Dagdelen, Eisele, Trowitzsch-Kienast, Zeitz, Jia, Stickel, Somasundaram: "Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-beta-mediated profibrotic gene expression pattern." in: The Journal of nutritional biochemistry, Vol. 20, Issue 8, pp. 597-606, (2009) (PubMed).

Target Details

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).