SensoLyte® Green Elastase Assay Kit

Catalog No. ABIN1882454

Quick Overview for SensoLyte® Green Elastase Assay Kit (ABIN1882454)

Target: Elastase

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® Green Elastase Assay Kit uses natural substrate elastin labeled with the 5-FAM fluorophore and the QXL™ 520 quencher. Proteolytic cleavage of labeled elastin yields brightly green fluorescence, which can be continuously monitored at excitation/emission= 488 nm/520 nm. Increase in fluorescence intensity is directly proportional to elastase activity. This kit does not require any separation steps and can be used to continuously measure the kinetics of elastase activity.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Component A from light and moisture.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.

Target Details

Target: Elastase

Background: Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.