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- Target See all Elastase Kits
- Elastase
- Detection Method
- Fluorometric
- Application
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 /520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.
- Top Product
- Discover our top product Elastase ELISA Kit
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SensoLyte® Green Elastase Assay Kit
FRET Fluorometric
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- Comment
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FRET-based Assay Kit
- Restrictions
- For Research Use only
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- Handling Advice
- Protect Component A from light and moisture.
- Storage
- -20 °C
- Storage Comment
- Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.
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- Target
- Elastase
- Abstract
- Elastase Products
- Background
- Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.
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