SensoLyte® 520 MMP-12 Assay Kit

Catalog No. ABIN1882514

Quick Overview for SensoLyte® 520 MMP-12 Assay Kit (ABIN1882514)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: SensoLyte® 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Yokose, Hachiya, Sriwiriyanont, Fujimura, Visscher, Kitzmiller, Bello, Tsuboi, Kitahara, Kobinger, Takema: "The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage." in: The Journal of investigative dermatology, Vol. 132, Issue 12, pp. 2800-9, (2012) (PubMed).

Haq, Lowrey, Kalsheker, Johnson: "Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD." in: Thorax, Vol. 66, Issue 11, pp. 970-6, (2011) (PubMed).

Margheri, Serratì, Lapucci, Chillà, Bazzichi, Bombardieri, Kahaleh, Calorini, Bianchini, Fibbi, Del Rosso: "Modulation of the angiogenic phenotype of normal and systemic sclerosis endothelial cells by gain-loss of function of pentraxin 3 and matrix metalloproteinase 12." in: Arthritis and rheumatism, Vol. 62, Issue 8, pp. 2488-98, (2010) (PubMed).

Target Details

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: MMP-12

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is expressed in activated macrophages and may play a role in smoke-induced emphysema.