SensoLyte® 520 MMP-8 Assay Kit

Catalog No. ABIN1882526

Product Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Schubert-Unkmeir, Konrad, Slanina, Czapek, Hebling, Frosch: "Neisseria meningitidis induces brain microvascular endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8." in: PLoS pathogens, Vol. 6, Issue 4, pp. e1000874, (2010) (PubMed).

Slanina, König, Claus, Frosch, Schubert-Unkmeir: "Real-time impedance analysis of host cell response to meningococcal infection." in: Journal of microbiological methods, Vol. 84, Issue 1, pp. 101-8, (2010) (PubMed).

Target Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Alternative Name: MMP-8 (MMP8 Products)

Synonyms: MMP8 Kit, BB138268 Kit, CLG1 Kit, HNC Kit, MMP-8 Kit, PMNL-CL Kit, matrix metallopeptidase 8 Kit, neutrophil collagenase Kit, MMP8 Kit, Mmp8 Kit, LOC100339790 Kit

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (netrophil collagnease) is proposed as a potential anti-cancer drug target.