CCL27 ELISA Kit

Catalog No. ABIN1889310

This Human CCL27 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CCL27.

Quick Overview for CCL27 ELISA Kit (ABIN1889310)

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Binding Specificity: AA 25-112

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details CCL27 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL27/CTACK

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: F25-G112

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: F25-G112

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Tissue Specificity: Testis, thymus, placenta, ovary and skin.

Plate: Pre-coated

Protocol: human CCL27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL27 has been precoated onto 96-well plates. Standards(E.coli, F25-G112) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL27 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL27 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 352, Standard deviation: 14.78, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 794, Standard deviation: 30.97, CV(%): 3.9
• Sample 3: n=16, Mean(pg/ml): 1456, Standard deviation: 77.17, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 376, Standard deviation: 31.58, CV(%): 8.4
• Sample 2: n=24, Mean(pg/ml): 822, Standard deviation: 64.94, CV(%): 7.9
• Sample 3: n=24, Mean(pg/ml): 1570, Standard deviation: 111.47, CV(%): 7.1

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CCL27

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Alternative Name: CCL27

Background:

Protein Function: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.

Background: Chemokine(C-C motif) ligand 27(CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine(ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine(CTACK). This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.

Synonyms: C-C motif chemokine 27,CC chemokine ILC,Cutaneous T-cell-attracting chemokine,CTACK,ESkine,IL-11 R-alpha-locus chemokine,Skinkine,Small-inducible cytokine A27,CCL27,ILC, SCYA27,

Full Gene Name: C-C motif chemokine 27

Cellular Localisation: Secreted.

Gene ID: 10850

UniProt: Q9Y4X3