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CXCL7 ELISA Kit

PPBP Reactivity: Human AA 59-128 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889316
  • Target See all CXCL7 (PPBP) ELISA Kits
    CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
    Binding Specificity
    AA 59-128
    Reactivity
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL7
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: A59-D128
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <2pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: A59-D128
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

    Plate
    Pre-coated
    Protocol
    human CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL7 has been precoated onto 96-well plates. Standards(E.coli, A59-D128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL7 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 85.6, Standard deviation: 3.85, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 227, Standard deviation: 12.7, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 519, Standard deviation: 30.1, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 82.8, Standard deviation: 6.13, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 236, Standard deviation: 14.6, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 527, Standard deviation: 37.4, CV(%): 7.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CXCL7 (PPBP) ELISA Kits
    CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
    Alternative Name
    CXCL7 (PPBP Products)
    Synonyms
    2400003M24Rik ELISA Kit, AI854500 ELISA Kit, CTAP3 ELISA Kit, CTAPIII ELISA Kit, Cxcl7 ELISA Kit, LA-PF4 ELISA Kit, LDGF ELISA Kit, MDGF ELISA Kit, NAP-2 ELISA Kit, NAP-2-L1 ELISA Kit, Scyb7 ELISA Kit, TGB ELISA Kit, TGB1 ELISA Kit, THBGB1 ELISA Kit, b-TG1 ELISA Kit, beta-TG ELISA Kit, B-TG1 ELISA Kit, Beta-TG ELISA Kit, CTAP-III ELISA Kit, CXCL7 ELISA Kit, PBP ELISA Kit, SCYB7 ELISA Kit, TC1 ELISA Kit, TC2 ELISA Kit, THBGB ELISA Kit, Nap-2 ELISA Kit, CTAPIII/NAP-2 ELISA Kit, Platelet basic protein ELISA Kit, pro-platelet basic protein ELISA Kit, scyb7 ELISA Kit, Ppbp ELISA Kit, PPBP ELISA Kit
    Background

    Protein Function: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .

    Background: Chemokine(C-X-C motif) ligand 7(CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

    Synonyms: Platelet basic protein,PBP,C-X-C motif chemokine 7,Leukocyte-derived growth factor,LDGF,Macrophage-derived growth factor,MDGF,Small-inducible cytokine B7,Connective tissue-activating peptide III,CTAP-III,LA-PF4,Low-affinity platelet factor IV,TC-2,Connective tissue-activating peptide III(1-81),CTAP-III(1-81),Beta-thromboglobulin,Beta-TG,Neutrophil-activating peptide 2(74),NAP-2(74),Neutrophil-activating peptide 2(73),NAP-2(73),Neutrophil-activating peptide 2,NAP-2,TC-1,Neutrophil-activating peptide 2(1-66),NAP-2(1-66),Neutrophil-activating peptide 2(1-63),NAP-2(1-63),PPBP,CTAP3, CXCL7, SCYB7, TGB1, THBGB1,

    Full Gene Name: Platelet basic protein

    Cellular Localisation: Secreted.
    Gene ID
    5473
    UniProt
    P02775
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