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TIMP2 ELISA Kit

TIMP2 Reactivity: Mouse AA 27-220 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Pubmed (6 references)
Catalog No. ABIN1889329
$499.00
Plus shipping costs $45.00
96 tests
Shipping to: United States
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  • Target See all TIMP2 ELISA Kits
    TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
    Binding Specificity
    AA 27-220
    Reactivity
    • 14
    • 7
    • 7
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TIMP-2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: C27-P220
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: C27-P220
    Featured
    Discover our best selling TIMP2 ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

    Tissue Specificity: Detected in testis, retina, hippocampus and cerebral cortex. .

    Plate
    Pre-coated
    Protocol
    mouse TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-2 has been precoated onto 96-well plates. Standards(NSO, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TIMP-2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TIMP-2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.24, Standard deviation: 0.056, CV(%): 4.5
    • Sample 2: n=16, Mean(ng/ml): 3.85, Standard deviation: 0.146, CV(%): 3.8
    • Sample 3: n=16, Mean(ng/ml): 6.85, Standard deviation: 0.219, CV(%): 3.2,
    • Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.088, CV(%): 6.7
    • Sample 2: n=24, Mean(ng/ml): 3.96, Standard deviation: 0.245, CV(%): 6.2
    • Sample 3: n=24, Mean(ng/ml): 6.88, Standard deviation: 0.516, CV(%): 7.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Papakonstantinou, Karakiulakis, Batzios, Savic, Roth, Tamm, Stolz: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." in: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).

    Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

    Cavdar, Ozbal, Celik, Ergur, Guneli, Ural, Camsari, Guner: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, (2014) (PubMed).

    Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

    Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

    Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." in: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

  • Target See all TIMP2 ELISA Kits
    TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
    Alternative Name
    TIMP2 (TIMP2 Products)
    Synonyms
    CSC-21K ELISA Kit, DDC8 ELISA Kit, TIMP-2 ELISA Kit, D11Bwg1104e ELISA Kit, Timp-2 ELISA Kit, etID32613.12 ELISA Kit, fa96h09 ELISA Kit, timp2 ELISA Kit, wu:fa96h09 ELISA Kit, zgc:136726 ELISA Kit, TIMP metallopeptidase inhibitor 2 ELISA Kit, tissue inhibitor of metalloproteinase 2 ELISA Kit, TIMP metallopeptidase inhibitor 2a ELISA Kit, TIMP2 ELISA Kit, Timp2 ELISA Kit, timp2a ELISA Kit
    Background

    Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

    Background: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

    Synonyms: Metalloproteinase inhibitor 2,Tissue inhibitor of metalloproteinases 2,TIMP-2,Timp2,Timp-2,

    Full Gene Name: Metalloproteinase inhibitor 2

    Cellular Localisation: Secreted.
    Gene ID
    21858
    UniProt
    P25785
    Pathways
    cAMP Metabolic Process
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