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TGFB2 ELISA Kit (Transforming Growth Factor, beta 2) ELISA Kit

TGFB2 Reactivity: Human AA 303-414 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant
Pubmed (2)
Catalog No. ABIN1889330
$369.00
Plus shipping costs $45.00
96 tests
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  • Target
    Binding Specificity
    AA 303-414
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human TGF-beta 2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: A303-S414
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Predicted Reactivity
    Bovine,Canine,Chicken,Horse,Monkey,Rabbit
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A303-S414
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the TGF-beta family.

    Plate
    Pre-coated
    Protocol
    human TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF-beta 2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGF-beta 2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 221, Standard deviation: 13.7, CV(%): 6.2
    • Sample 2: n=16, Mean(pg/ml): 564, Standard deviation: 13.6, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 1145, Standard deviation: 46.9, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 236, Standard deviation: 15.8, CV(%): 6.7
    • Sample 2: n=24, Mean(pg/ml): 576, Standard deviation: 33.41, CV(%): 5.8
    • Sample 3: n=24, Mean(pg/ml): 1095, Standard deviation: 70.1, CV(%): 6.4
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Song, Zhang, Zhang, Du, Guo, Kuang, Wang, Wu, Zou, Zou, Lv, Wang: "A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting." in: Scientific reports, Vol. 8, Issue 1, pp. 6394, 2018 (PubMed).

    Li, Liu, Shen, Wang, Chen, Xu, Wen: "MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." in: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, 2014 (PubMed).

  • Target
    Alternative Name
    TGFB2 (TGFB2 ELISA Kit Abstract)
    Synonyms
    LDS4, TGF-beta2, tgf-beta2, tgfb2-A, BB105277, Tgf-beta2, Tgfb-2, TGFB2, TGFbeta2, MGF, TGF-B2, transforming growth factor beta 2, transforming growth factor beta 2 L homeolog, transforming growth factor, beta 2, TGFB2, tgfb2.L, Tgfb2, tgfb2
    Background

    Protein Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

    Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

    Synonyms: Transforming growth factor beta-2,TGF-beta-2,BSC-1 cell growth inhibitor,Cetermin,Glioblastoma-derived T-cell suppressor factor,G-TSF,Polyergin,Latency-associated peptide,LAP,TGFB2,

    Full Gene Name: Transforming growth factor beta-2

    Cellular Localisation: Secreted.
    Gene ID
    7042
    UniProt
    P61812
    Pathways
    Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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