Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Periostin ELISA Kit

POSTN Reactivity: Mouse AA 24-836 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889359
  • Target See all Periostin (POSTN) ELISA Kits
    Periostin (POSTN)
    Binding Specificity
    AA 24-836
    Reactivity
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    93.7-6000 pg/mL
    Minimum Detection Limit
    93.7 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Periostin/OSF2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: N24-Q836
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: N24-Q836
    Featured
    Discover our best selling POSTN ELISA Kit
    Top Product
    Discover our top product POSTN ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 EMI domain.

    Tissue Specificity: Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .

    Plate
    Pre-coated
    Protocol
    mouse Periostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Periostin has been precoated onto 96-well plates. Standards(NSO, N24-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Periostin amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m mouse Periostin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Periostin standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 783, Standard deviation: 49.33, CV(%): 6.3
    • Sample 2: n=16, Mean(pg/ml): 1721, Standard deviation: 98.1, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 2910, Standard deviation: 142.6, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 867, Standard deviation: 63.3, CV(%): 7.3
    • Sample 2: n=24, Mean(pg/ml): 1834, Standard deviation: 143.1, CV(%): 7.8
    • Sample 3: n=24, Mean(pg/ml): 3127, Standard deviation: 172, CV(%): 5.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Krencik, Hokanson, Narayan, Dvornik, Rooney, Rauen, Weiss, Rowitch, Ullian: "Dysregulation of astrocyte extracellular signaling in Costello syndrome." in: Science translational medicine, Vol. 7, Issue 286, pp. 286ra66, (2015) (PubMed).

  • Target See all Periostin (POSTN) ELISA Kits
    Periostin (POSTN)
    Alternative Name
    POSTN (POSTN Products)
    Synonyms
    fa99h07 ELISA Kit, pn ELISA Kit, postn ELISA Kit, wu:fa99h07 ELISA Kit, wu:fc70f09 ELISA Kit, zgc:153873 ELISA Kit, LOC100008945 ELISA Kit, osf-2 ELISA Kit, pdlpostn ELISA Kit, periostin ELISA Kit, OSF-2 ELISA Kit, OSF2 ELISA Kit, PDLPOSTN ELISA Kit, PN ELISA Kit, RP11-412K4.1 ELISA Kit, A630052E07Rik ELISA Kit, AI747096 ELISA Kit, Osf2 ELISA Kit, PLF ELISA Kit, peri ELISA Kit, Plf ELISA Kit, periostin ELISA Kit, periostin, osteoblast specific factor b ELISA Kit, periostin, osteoblast specific factor ELISA Kit, POSTN ELISA Kit, postnb ELISA Kit, postn ELISA Kit, Postn ELISA Kit
    Background

    Protein Function: Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .

    Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

    Synonyms: Periostin,PN,Osteoblast-specific factor 2,OSF-2,Postn,Osf2,

    Full Gene Name: Periostin

    Cellular Localisation: Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.
    Gene ID
    50706
    UniProt
    Q62009
You are here:
Support