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Betacellulin ELISA Kit

This Mouse Betacellulin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Betacellulin in Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum.
Catalog No. ABIN1889364

Quick Overview for Betacellulin ELISA Kit (ABIN1889364)

Target

See all Betacellulin (BTC) ELISA Kits
Betacellulin (BTC)

Binding Specificity

AA 32-111

Reactivity

  • 5
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Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

7.8-500 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Minimum Detection Limit

    7.8 pg/mL

    Purpose

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Betacellulin/BTC

    Brand

    PicoKine™

    Analytical Method

    Quantitative

    Specificity

    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    <10pg/mL

    Material not included

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111
  • Application Notes

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Comment

    Tissue Specificity: Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.

    Plate

    Pre-coated

    Protocol

    mouse BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BTC amount of sample captured in plate.

    Assay Procedure

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse BTC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse BTC standard solution and each sample is measured in duplicate.

    Assay Precision

    • Sample 1: n=16, Mean(pg/ml): 28.6, Standard deviation: 1.40, CV(%): 4.9
    • Sample 2: n=16, Mean(pg/ml): 69.4, Standard deviation: 3.61, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 279, Standard deviation: 15.90, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 29.3, Standard deviation: 1.70, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 69.8, Standard deviation: 4.40, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 285, Standard deviation: 23.09, CV(%): 8.1

    Restrictions

    For Research Use only
  • Handling Advice

    Avoid multiple freeze-thaw cycles.

    Storage

    -20 °C,4 °C

    Storage Comment

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Expiry Date

    12 months
  • Target See all Betacellulin (BTC) ELISA Kits

    Betacellulin (BTC)

    Alternative Name

    BTC

    Background

    Protein Function: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .

    Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

    Synonyms: Probetacellulin,Betacellulin,BTC,Btc,Bcn,

    Full Gene Name: Probetacellulin

    Cellular Localisation: Betacellulin: Secreted, extracellular space.

    Gene ID

    12223

    UniProt

    Q05928

    Pathways

    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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