CD320 ELISA Kit

Catalog No. ABIN1889407

Human CD320 ELISA Kit Colorimetric assay for quantification of Human CD320.

Quick Overview for CD320 ELISA Kit (ABIN1889407)

Target: CD320 (CD320 Molecule (CD320))

Binding Specificity: AA 36-229

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Product Details CD320 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD320/8D6A

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S36-Y229

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S36-Y229

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed abundantly on follicular dendritic cells (FDCs). .

Plate: Pre-coated

Protocol: human CD320 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD320 has been precoated onto 96-well plates. Standards(NSO, S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD320 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD320 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD320 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD320 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.042, CV(%): 3.8
• Sample 2: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.14, CV(%): 4.5
• Sample 3: n=16, Mean(ng/ml): 6.3, Standard deviation: 0.31, CV(%): 4.9,
• Sample 1: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.08, CV(%): 6.2
• Sample 2: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.194, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 5.9, Standard deviation: 0.313, CV(%): 5.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CD320

Target: CD320 (CD320 Molecule (CD320))

Alternative Name: CD320

Background:

Protein Function: Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. .

Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

Synonyms: CD320 antigen,8D6 antigen,FDC-signaling molecule 8D6,FDC-SM-8D6,Transcobalamin receptor,TCblR,CD320,CD320,8D6A,UNQ198/PRO224,

Full Gene Name: CD320 antigen

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 51293

UniProt: Q9NPF0