Betacellulin ELISA Kit

Catalog No. ABIN1889437

Rat Betacellulin ELISA Kit Colorimetric assay for quantification of Rat Betacellulin in Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Quick Overview for Betacellulin ELISA Kit (ABIN1889437)

Target: Betacellulin (BTC)

Binding Specificity: AA 32-111

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details Betacellulin ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat BTC

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: D32-Y111

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: D32-Y111

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BTC amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat BTC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat BTC standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 3.61, CV(%): 3.8
• Sample 2: n=16, Mean(pg/ml): 178, Standard deviation: 7.3, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 362, Standard deviation: 16.29, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 4.62, CV(%): 4.4
• Sample 2: n=24, Mean(pg/ml): 241, Standard deviation: 12.53, CV(%): 5.2
• Sample 3: n=24, Mean(pg/ml): 413, Standard deviation: 23.13, CV(%): 5.6

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Betacellulin

Target: Betacellulin (BTC)

Alternative Name: BTC

Background:

Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Synonyms: Betacellulin ,Protein Btc ,Btc ,BTC ,

Full Gene Name: betacellulin

Gene ID: 64022

UniProt: Q9JJM4

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway