CAPN10 ELISA Kit

Catalog No. ABIN7540316

This Chemical CAPN10 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Chemical CAPN10.

Quick Overview for CAPN10 ELISA Kit (ABIN7540316)

Target: CAPN10 (Calpain 10 (CAPN10))

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 1-243 pM/mL

Application: ELISA

Sample Type: Serum, Plasma, Saliva, Cell Culture Supernatant, Urine

Product Details CAPN10 ELISA Kit

Minimum Detection Limit: 1 pM/mL

Purpose: Quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

Analytical Method: Quantitative

Specificity: Easy - use: All reagents and buffers for cAMP test are provided.

Cross-Reactivity (Details): No significant cross-reactivity of similar compounds was found

Sensitivity: 0.61 pmol/mL

Characteristics: cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Components: Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cAMP mAb: 12 ml
HRP-cAMP: 6 ml
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml): 1.5 ml
cAMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy

Application Details

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Restrictions: For Research Use only

Handling

Handling Advice: Do not freeze the kit.

Storage: 4 °C

Storage Comment: The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeks at 2-8 °C.

Expiry Date: 12 months

Target Details for CAPN10

Target: CAPN10 (Calpain 10 (CAPN10))

Alternative Name: cAMP / Cyclic AMP

Target Type: Chemical

Background: CAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases.

Pathways: Positive Regulation of Peptide Hormone Secretion