NOTCH2NL ELISA Kit

Catalog No. ABIN2041984

Human NOTCH2NL ELISA Kit, Colorimetric assay for quantification of Human NOTCH2NL.

Quick Overview for NOTCH2NL ELISA Kit (ABIN2041984)

Target: NOTCH2NL (Notch 2 N-terminal Like (NOTCH2NL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details NOTCH2NL ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Notch2 N-Terminal Like Protein (NOTCH2NL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch2 N-Terminal Like Protein (NOTCH2NL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch2 N-Terminal Like Protein (NOTCH2NL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch2 N-Terminal Like Protein (NOTCH2NL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Notch2 N-Terminal Like Protein (NOTCH2NL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NOTCH2NL

Target: NOTCH2NL (Notch 2 N-terminal Like (NOTCH2NL))

Alternative Name: Notch2 N-Terminal Like Protein (NOTCH2NL)