USP6NL ELISA Kit
Quick Overview for USP6NL ELISA Kit (ABIN2041986)
Target
See all USP6NL ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.058 ng/mL
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Plate
- Pre-coated
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Protocol
- USP6 N-Terminal Like Protein (USP6NL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to USP6 N-Terminal Like Protein (USP6NL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to USP6 N-Terminal Like Protein (USP6NL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain USP6 N-Terminal Like Protein (USP6NL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of USP6 N-Terminal Like Protein (USP6NL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- USP6NL (USP6 N-terminal Like (USP6NL))
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Alternative Name
- USP6 N-Terminal Like Protein (USP6NL)
Target See all USP6NL ELISA Kits
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