PRSS23 ELISA Kit
Quick Overview for PRSS23 ELISA Kit (ABIN2042308)
Target
See all PRSS23 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.31 ng/mL
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Sensitivity
- < 0.123 ng/mL
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Plate
- Pre-coated
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Protocol
- Protease, Serine 23 (PRSS23) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 23 (PRSS23). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 23 (PRSS23). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 23 (PRSS23), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease, Serine 23 (PRSS23) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PRSS23 (Protease, Serine, 23 (PRSS23))
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Alternative Name
- Protease, Serine 23 (PRSS23)
Target See all PRSS23 ELISA Kits
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