PRSS50 ELISA Kit

Catalog No. ABIN2042312

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PRSS50.

Quick Overview for PRSS50 ELISA Kit (ABIN2042312)

Target: PRSS50 (Protease, serine, 50 (PRSS50))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details PRSS50 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protease, Serine 50 (PRSS50) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 50 (PRSS50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 50 (PRSS50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 50 (PRSS50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease, Serine 50 (PRSS50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PRSS50

Target: PRSS50 (Protease, serine, 50 (PRSS50))

Alternative Name: Protease, Serine 50 (PRSS50)