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ANG ELISA Kit

Human ANG ELISA Kit Colorimetric assay for quantification of Human ANG.
Catalog No. ABIN2042344

Quick Overview for ANG ELISA Kit (ABIN2042344)

Target

See all ANG ELISA Kits
ANG (Angiogenin (ANG))

Reactivity

  • 11
  • 5
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

8-5000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    8 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 12 pg/mL

    Components

    96-well plate pre-coated with anti-ANG, Human
    Lyophilized recombinant ANG, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human ANG in cell culture supernates, serum and plasma (heparin, EDTA).Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene.[1] Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease.[2] Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all ANG ELISA Kits

    ANG (Angiogenin (ANG))

    Alternative Name

    ANG

    Gene ID

    283

    UniProt

    P03950
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