ANG ELISA Kit

Catalog No. ABIN921052

Human ANG ELISA Kit, Colorimetric assay for quantification of Human ANG, that has been mentioned in 4+ publications.

Quick Overview for ANG ELISA Kit (ABIN921052)

Target: ANG (Angiogenin (ANG))

Binding Specificity: AA 25-147

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details ANG ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Sandwich Human Angiogenin/ANG ELISA Kit to quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: Q25-P147

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 12 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.5%, 6.7%, 6.7%
Inter-Assay Precision (CV%): 4.6%, 6.8%, 8.5%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for ANG ELISA Kit (ABIN921052)

Shi, Zhang, Fu, Lu, Ye, Xiao: "Angiotensin II as a morphogenic cytokine stimulating fibrogenesis of human tenon's capsule fibroblasts." in: Investigative ophthalmology & visual science, Vol. 56, Issue 2, pp. 855-64, (2015) (PubMed).

Bai, Li, Tian, Zhou: "Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes." in: PLoS ONE, Vol. 9, Issue 4, pp. e96117, (2014) (PubMed).

Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, (2013) (PubMed).

Jerosch, Castro, Colemont: "An isolated reactive synovitis of the sternoclavicular joint." in: Acta orthopaedica Belgica, Vol. 55, Issue 2, pp. 222-4, (1989) (PubMed).

Target Details for ANG

Target: ANG (Angiogenin (ANG))

Alternative Name: ANG

Background:

Background: Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Gene Full Name: angiogenin

Gene ID: 283

UniProt: P03950