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Angiopoietin 1 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Angiopoietin 1.
Catalog No. ABIN2042345

Quick Overview for Angiopoietin 1 ELISA Kit (ABIN2042345)

Target

See all Angiopoietin 1 (ANGPT1) ELISA Kits
Angiopoietin 1 (ANGPT1)

Reactivity

  • 14
  • 7
  • 7
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

6-10000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-Angiopoietin-1, Human
    Lyophilized recombinant Angiopoietin-1, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human Angiopoietin-1 in cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk.Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all Angiopoietin 1 (ANGPT1) ELISA Kits

    Angiopoietin 1 (ANGPT1)

    Alternative Name

    Angiopoietin-1

    Gene ID

    284

    UniProt

    Q15389

    Pathways

    RTK Signaling, Glycosaminoglycan Metabolic Process
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