Angiopoietin 2 ELISA Kit

Catalog No. ABIN2042346

This Human Angiopoietin 2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Angiopoietin 2.

Quick Overview for Angiopoietin 2 ELISA Kit (ABIN2042346)

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details Angiopoietin 2 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Angiopoietin-2, Human
Lyophilized recombinant Angiopoietin-2, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Angiopoietin-2 in cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk.Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling dorsal aorta and major aortic branches, ovary, placenta and uterus.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin-2

Gene ID: 285

UniProt: O15123

Pathways: RTK Signaling