Angiopoietin 2 ELISA Kit

Catalog No. ABIN2042347

Mouse Angiopoietin 2 ELISA Kit Colorimetric assay for quantification of Mouse Angiopoietin 2.

Quick Overview for Angiopoietin 2 ELISA Kit (ABIN2042347)

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details Angiopoietin 2 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Angiopoietin-2, Mouse
Lyophilized recombinant Angiopoietin-2, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse Angiopoietin-2 in cell culture supernates, serum and plasma (heparin, EDTA).Angiopoietin-2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. Human and mouse ANGPT2 are 85% identical in amino acid sequence and approximately 60% identical to their ANGPT1 homologs. Transgenic overexpression of ANGPT2 in mice disrupted blood vessel formation in the mouse embryo. By FISH and radiation hybrid analysis, the human ANGPT2 gene was mapped to 8p23, and in a region of syntenic homology on mouse chromosome 8, where the Angpt2 gene was mapped. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis .

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin-2

Gene ID: 11601

UniProt: B9EHQ4

Pathways: RTK Signaling